Introduction. Effects of psychological stress on blood hypercoagulation have been shown by a number of authors. In healthy subjects acute mental stress simultaneously activates coagulation and fibrinolysis within a physiological range. However, in patients with atherosclerosis or in healthy subjects under acute or chronic psychosocial stressors (e.g. exam, earthquake, blood donation, job strain, low socioeconomic status, social isolation) hypercoagulable states reflected by an increased number of procoagulant molecules and by reduced fibrinolytic capacity might develop. There is also evidence that points to hypercoagulability in depression. Aim. The aim of this paper was to demonstrate the positive effect of emotional expression on blood coagulation using dark field microscopy. Materials and methods. Cases of three patients suffering from psychological stress are presented. Humaniversity therapy was applied for all cases integrating a variety of therapeutic techniques, such as: emotional expression, cognitive change, integrity of a holistic approach, touch and positivity. Dark field microscopy was used before and after the sessions to evaluate the effect of therapeutic work on blood coagulation. Results and Discussion. The results of three cases showed the immediate effect of emotional release on blood coagulation. After the sessions lasting from 1–1.5 hours, all three cases reported improved emotional well-being with changes in blood clotting from levels 4 and 5 (light severe and severe clotting) to no clotting at all. Conclusions. A positive objective effect of emotional expression on blood coagulation can be demonstrated using dark field microscopy.