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Expression levels of PDYN and OPRM1 genes in SH-SY5Y cells exposed to 50 Hz electromagnetic field
1
Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran
Submission date: 2017-07-04
Acceptance date: 2017-09-18
Online publication date: 2018-05-28
Publication date: 2019-10-31
Corresponding author
Mostafa Saadat
Department of Biology, College of Sciences, Shiraz University, Shiraz 71467-13565, Iran. Tel: +9871 36137432; Fax: +9871 32280916.
Department of Biology, College of Sciences, Shiraz University, Shiraz 71467-13565, Iran. Tel: +9871 36137432; Fax: +9871 32280916.
Pol. Ann. Med. 2019;26(1):36-40
KEYWORDS
ABSTRACT
Introduction:
Extremely low-frequency (ELF) (<300 Hz) electromagnetic fields (EMFs) may significantly affect several biological processes at the cellular and molecular level. Considering that ELF-EMF is abundant in our environment and associated with reactive oxygen species (ROS) production, exposure to EMF should be considered as a public health issue. ELF-EMF may alter the mRNA expression levels of several genes. Prodynorphin (PDYN, OMIM: 131340), precursor of several endogenous opioid neuropeptides, and opioid receptor mu-1 (OPRM1, OMIM: 600018) a member of opioid receptor family, are associated with nociception and drug-dependency.
Aim:
This study was conducted to elucidate the effects of ELF-EMF on expression levels of PDYN and OPRM1.
Material and methods:
Human SH-SY5Y cells were exposed first to EMF and harvested at three time points post exposure; immediately after exposure (0h), 2h and 4h after exposure. The 0.50 mT intensity of 50 Hz EMF and two exposure conditions (‘15 min field-on/15 min field-off’ and ‘30 min field-on continuously’) were used. Using quantitative real-time PCR, the relative PDYN and OPRM1 mRNA expression levels were calculated.
Results and discussion:
After continuous exposure to ELF-EMF, analysis of variance revealed a significant reduction of PDYN mRNA expression levels at 0 hours and 2 hours time points (F = 23.86; df = 3, 8; P < 0.001). The OPRM1 mRNA expression levels did not show any significant alteration between the examined conditions.
Conclusions:
In the present study the continuous exposure condition of ELF-EMF was associated with the lower expression levels of the PDYN.
Extremely low-frequency (ELF) (<300 Hz) electromagnetic fields (EMFs) may significantly affect several biological processes at the cellular and molecular level. Considering that ELF-EMF is abundant in our environment and associated with reactive oxygen species (ROS) production, exposure to EMF should be considered as a public health issue. ELF-EMF may alter the mRNA expression levels of several genes. Prodynorphin (PDYN, OMIM: 131340), precursor of several endogenous opioid neuropeptides, and opioid receptor mu-1 (OPRM1, OMIM: 600018) a member of opioid receptor family, are associated with nociception and drug-dependency.
Aim:
This study was conducted to elucidate the effects of ELF-EMF on expression levels of PDYN and OPRM1.
Material and methods:
Human SH-SY5Y cells were exposed first to EMF and harvested at three time points post exposure; immediately after exposure (0h), 2h and 4h after exposure. The 0.50 mT intensity of 50 Hz EMF and two exposure conditions (‘15 min field-on/15 min field-off’ and ‘30 min field-on continuously’) were used. Using quantitative real-time PCR, the relative PDYN and OPRM1 mRNA expression levels were calculated.
Results and discussion:
After continuous exposure to ELF-EMF, analysis of variance revealed a significant reduction of PDYN mRNA expression levels at 0 hours and 2 hours time points (F = 23.86; df = 3, 8; P < 0.001). The OPRM1 mRNA expression levels did not show any significant alteration between the examined conditions.
Conclusions:
In the present study the continuous exposure condition of ELF-EMF was associated with the lower expression levels of the PDYN.
FUNDING
This study was supported by Shiraz University, Iran.
CONFLICT OF INTEREST
No competing interests are declared by any of the authors.
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